PCR Techniques

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A schematic summary of the PCR reaction

A schematic summary of the PCR reaction

Term: 2014
Published: February 26, 2014
Revised: March 28, 2014

What is PCR and where can it be used

The polymerase chain reaction (PCR) is a test tube system for DNA replication which allows a “target” DNA sequence to be selectively amplified several million-fold in just a few hours. The PCR achieves amplification of a predetermined fragment of DNA, (the target; which can e.g. be from 100 – 1000 bp long) with the apparent disadvantage that the sequences flanking the target region must be known, the latter precludes the use of PCR from analysis of DNA regions that have not previously been studied by standard methods. 

About The Instructor

Prof Estelle Venter

Prof Estelle Venter

  • BSc, MSc, PhD
  • Professor: Department of Veterinary Tropical Diseases, Faculty of Veterinary Science, University of Pretoria, South Africa
Creative Commons Attribution 4.0 International
This Work, PCR Techniques, by Prof Estelle Venter is licensed under a Creative Commons Attribution license.