Real-time PCR

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TaqMan® probe reaction

TaqMan® probe reaction

Term: 2014
Published: February 26, 2014
Revised: March 28, 2014

Polymerase chain reaction (PCR) and DNA probe-based techniques have improved the sensitivity and specificity that previous diagnostic tests lacked. However, PCR and probing assays are relatively time-consuming and labour intensive, particularly when separate hybridization steps are required to confirm test outcomes.

Recently, real-time PCR technology has greatly improved molecular detection of organisms of veterinary, medical and economic importance. The real-time PCR technology employs similar amplification processes followed in traditional PCR methods (denaturing, annealing and extension) however, the real-time PCR technology allows fast real-time monitoring of a PCR reaction. Amplification and accurate detection of specific targeted nucleic acid sequences is accomplished in a single closed tube, without the need for post-amplification analysis.

About The Instructor

Dr Kgomotso Sibeko-Matjila

Dr Kgomotso Sibeko-Matjila

  • BSc, MSc, PhD
  • Senior lecturer: Department of Veterinary Tropical Diseases, Faculty of Veterinary Science, University of Pretoria, South Africa
Creative Commons Attribution 4.0 International
This Work, Real-time PCR, by Dr Kgomotso Sibeko-Matjila is licensed under a Creative Commons Attribution license.